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Liver Veal Egg Yolk Agar (BAM M84)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Veal Egg Yolk Agar

(BAM M84)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1080.0mL:

Liver veal agar ................................................... 1.0L

Egg yolk emulsion, 50%............................... 80.0mL

pH 7.3 ± 0.2 at 25°C

Liver Veal Agar:

Composition per liter:

Veal, infusion from .........................................500.0g

Beef liver, infusion from...................................50.0g

Gelatin...............................................................20.0g

Proteose peptone...............................................20.0g

Agar ..................................................................15.0g

Soluble starch....................................................10.0g

Glucose ...............................................................5.0g

NaCl....................................................................5.0g

Casein .................................................................2.0g

NaNO3 ................................................................2.0g

Enzymatic digest of protein................................1.3g

Pancreatic digest of casein..................................1.3g

Source: This medium is available as a premixed

powder from BD Diagnostic Systemss.

Preparation of Liver Veal Agar: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Gently heat and bring to

boiling. Distribute into tubes or flasks. Make sure

that some liver and veal particles are transferred to

each tube. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C.

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

NaCl (0.9% solution) .................................... 50.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Preparation of Medium: To 1.0L of cooled ster

ile liver veal agar, aseptically add 80.0mL of sterile

egg yolk emulsion, 50%. Mix thoroughly. Pour into

sterile Petri dishes. Dry plates at 35°C for 24 hr.

Use: For the cultivation of a variety of anaerobic or

ganisms.

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Liver Veal Egg Yolk Agar (BAM M84)