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Litmus Milk

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Litmus Milk

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Skim milk........................................................100.0g

Azolitmin ............................................................0.5g

Na2SO3................................................................0.5g

pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 20 min

at 10 psi pressure–115°C.

Use: For the maintenance of lactic acid bacteria and

for the differentiation of several bacteria, especially

Clostridium species, based on their action on milk.

Bacteria that do not ferment carbohydrates, such as

Proteus vulgaris or Moraxella lacunata, show no

change in the litmus indicator. Bacteria that ferment

lactose or glucose with the production of gas, such as

Clostridium perfringens, turn the medium pink and

frothy. Bacteria that proteolytically degrade lactalbu

min turn the medium blue. Bacteria that coagulate

casein form a curd or clot. Bacteria that peptonize

casein, such as Pseudomonas aeruginosa, show a

dissolution of the clot.