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Litmus Lactose Agar with Crystal Violet (LLK Agar)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Litmus Lactose Agar with Crystal Violet

(LLK Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

Lactose..............................................................10.0g

Meat peptone.......................................................5.0g

Beef extract.........................................................3.0g

Litmus .................................................................1.0g

Crystal Violet ...................................................5.0mg

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the maintenance of lactic acid bacteria and

for the differentiation of several bacteria on the basis

of lactose fermentation. Bacteria that ferment lactose

appear as red colonies. Bacteria that do not ferment

lactose appear as dark blue-purple colonies.