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Leptospira Medium, Modified

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ....................................................................1.5g

NaCl....................................................................0.5g

Peptone................................................................0.3g

Beef extract.........................................................0.2g

Hemin solution................................................ 2.5mL

Sterile rabbit serum..................................... 100.0mL

pH 7.3 ± 0.1 at 25°C

Hemin Solution:

Composition per 100.0mL:

Hemin................................................................0.05g

NaOH (1N solution)........................................ 1.0mL

Preparation of Hemin Solution: Add hemin to

1.0mL of 1N NaOH solution. Mix thoroughly. Bring

volume to 100.0mL with distilled/deionized water.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Preparation of Medium: Add components, ex

cept hemin solution and rabbit serum, to distilled/

deionized water and bring volume to 897.5mL. Mix

thoroughly. Gently heat and bring to boiling. Adjust

pH to 7.4. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 2.5mL of

sterile hemin solution and 100.0mL of sterile rabbit

serum. Mix thoroughly. The pH of the medium

should be 7.3. Store at 4°C for 24 hr. Inactivate me

dium at 56°C for 60 min. Aseptically distribute into

sterile tubes or flasks.

Use: For the cultivation and maintenance of Leptospi

ra biflexa, Leptospira borgpetersenii, Leptospira in

terrogans, Leptospira meyeri, Leptospira noguchii,

Leptospira santarosai, and Leptospira weili.