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Leptospira Medium, EMJH (Leptospira Medium, Ellinghausen-McCullough/ Johnson-Harris)

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Leptospira Medium, EMJH

(Leptospira Medium,

Ellinghausen-McCullough/

Johnson-Harris)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4.............................................................1.0g

NaCl....................................................................1.0g

KH2PO4...............................................................0.3g

NH4Cl ...............................................................0.25g

Thiamine..........................................................5.0mg

Rabbit serum............................................... 100.0mL

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept rabbit serum, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C. Aseptically add

sterile rabbit serum. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Lep

tospira species.