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Lauryl Sulfate Broth, Fluorocult (Fluorocult Lauryl Sulfate Broth)

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Lauryl Sulfate Broth, Fluorocult

(Fluorocult Lauryl Sulfate Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................20.0g

Lactose................................................................5.0g

NaCl....................................................................5.0g

K2HPO4.............................................................2.75g

KH2PO4.............................................................2.75g

L-tryptophan..........................................................1.g

Sodium lauryl sulfate..........................................0.1g

4-Methylumbelliferyl-ß-D-glucuronide..............0.1g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Cool. Dis

tribute into into test tubes containing inverted Durham

tubes. Autoclave for 15 min at 15 psi pressure–121°C.

The prepared broth is clear and yellowish-brown.

Use: For the detection of E. coli in milk. The medi

um complies with the German-DIN-Norm 10183 for

the examination of milk, with the regulations acc. to

§ 35 LMBG (01.00/54) for the examination of food,

and according to ISO/DIS 11886-2.2 (1994) for milk

and milk products. The lauryl sulfate largely inhibits

the growth of undesirable microbial flora. The pres

ence of E. coli is indicated by fluorescence under a

long wavelength UV lamp. A positive indole reaction

and gas formation due to fermentation of lactose con

firm the results.