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Lactobacillus Medium III

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Fructose...............................................................7.0g

Glucose ...............................................................7.0g

Maltose................................................................7.0g

Meat extract ........................................................5.0g

Sodium acetate·3H2O..........................................5.0g

Yeast extract........................................................5.0g

K2HPO4·3H2O.....................................................2.6g

Diammonium citrate ...........................................2.0g

Sodium gluconate ...............................................2.0g

L-Cysteine·HCl ...................................................0.5g

MgSO4·7H2O ......................................................0.1g

MnSO4·4H2O ....................................................0.05g

Tween 80......................................................... 1.0mL

pH 6.3 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH to 6.3. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus species.