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Lactobacillus Medium II (DSMZ Medium 93)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactobacillus Medium II

(DSMZ Medium 93)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Na-acetate .........................................................20.0g

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

Yeast extract........................................................5.0g

Meat extract ........................................................2.0g

KH2PO4...............................................................0.5g

K2HPO4...............................................................0.5g

MgSO4·7H2O ......................................................0.2g

DL-mevalonic acid..........................................30.0mg

FeSO4·7H2O...................................................10.0mg

MnSO4·2H2O ...................................................7.5mg

Ethanol.......................................................... 40.0mL

Tween 80......................................................... 1.0mL

pH 5.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and maintenance of Lactoba

cillus fructivorans and Lactobacillus homohiochii.