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Lactobacillus Medium

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Sodium acetate..................................................20.0g

Tryptone............................................................10.0g

Yeast extract........................................................5.0g

Beef extract.........................................................2.0g

K2HPO4 ..............................................................0.5g

KH2PO4 ..............................................................0.5g

MgSO4·7H2O...............................................200.0mg

FeSO4·7H2O ..................................................10.0mg

MnSO4·H2O.....................................................7.5mg

Ethanol.......................................................... 40.0mL

Mevalonic acid solution................................ 10.0mL

Tween 80......................................................... 1.0mL

pH 5.2 ± 0.2 at 25°C

Mevalonic Acid Solution:

Composition per 10.0mL:

DL-Mevalonic acid.........................................30.0mg

Preparation of Mevalonic Acid Solution: Add

DL-mevalonic acid to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Preparation of Medium: Add components, ex

cept mevalonic acid solution and ethanol, to distilled/

deionized water and bring volume to 950.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 40.0mL of filter-sterilized

ethanol and 10.0mL of sterile mevalonic acid solu

tion. Mix thoroughly. Aseptically distribute into ster

ile tubes or flasks.

Use: For the cultivation of Lactobacillus homohiochii.