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Lactobacillus Medium (ATCC Medium 1006)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactobacillus Medium

(ATCC Medium 1006)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Pancreatic digest of casein................................10.0g

Glucose ...............................................................5.0g

Yeast extract........................................................5.0g

KH2PO4...............................................................3.0g

K2HPO4...............................................................3.0g

Tryptose ..............................................................3.0g

Sodium acetate....................................................1.0g

L-Cysteine·HCl·H2O............................................0.2g

Salt solution R................................................. 5.0mL

Tween 80......................................................... 1.0mL

pH 6.3 ± 0.2 at 25°C

Salt Solution R:

Composition per 100.0mL:

MgSO4·7H2O ....................................................11.5g

MnSO4·2H2O ......................................................2.4g

FeSO4·7H2O......................................................0.68g

Preparation of Salt Solution R: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Store at 4°C.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and maintenance of Lacto

bacillus delbrueckii and Lactobacillus jensenii.