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Lactobacillus Heteroferm Screen Broth (MRS, Modified)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactobacillus Heteroferm Screen Broth

(MRS, Modified)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Proteose peptone No. 3.....................................10.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................5.0g

2-Phenylethyl alcohol .........................................3.0g

Ammonium citrate..............................................2.0g

K2HPO4...............................................................2.0g

MgSO4·7H2O......................................................0.1g

MnSO4·H2O......................................................0.05g

Bromcresol Green.............................................0.04g

Cycloheximide.................................................4.0mg

Tween 80......................................................... 1.0mL

pH 4.3 ± 0.01 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 4.3 with concentrated

HCl. Distribute into test tubes that contain an invert

ed Durham tube. Autoclave for 15 min at 15 psi pres

sure–121°C.

Use: For the isolation and cultivation of Lactobacil

lus species from foods.