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Lactobacillus Heteroferm Screen Agar (MRS, Modified)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactobacillus Heteroferm Screen Agar

(MRS, Modified)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Proteose peptone No. 3.....................................10.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................5.0g

2-Phenylethyl alcohol .........................................3.0g

Ammonium citrate..............................................2.0g

K2HPO4...............................................................2.0g

MgSO4·7H2O......................................................0.1g

MnSO4·H2O......................................................0.05g

Bromcresol Green.............................................0.04g

Cycloheximide.................................................4.0mg

Tween 80......................................................... 1.0mL

pH 5.5 ± 0.01 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°C. Adjust pH to 5.5 with gla

cial acetic acid. Pour into sterile Petri dishes.

Use: For the isolation and cultivation of Lactobacil

lus species from salad dressings.