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Kreb’s Yeast Lactate Medium

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract......................................................10.0g

Na2HPO4·2H2O...................................................3.0g

KH2PO4...............................................................1.0g

Sodium lactate solution................................. 40.0mL

pH 7.0 ± 0.2 at 25°C

Sodium Lactate Solution:

Composition per 100.0mL:

Sodium lactate...................................................70.0g

Preparation of Sodium Lactate Solution: Add

sodium lactate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept sodium lactate solution, to distilled/deionized wa

ter and bring volume to 960.0mL. Mix thoroughly.

Bring pH to 7.0. Autoclave for 15 min at 15 psi pres

sure–121°C. Aseptically add 40.0mL of sterile sodium

lactate solution. Mix thoroughly. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the cultivation of Propionibacterium acid

ipropionici, Propionibacterium freudenreichii, Pro

pionibacterium intermedium, Propionibacterium

jensenii, Propionibacterium species, and Propioni

bacterium thoenii.