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Kranep Agar Base

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Kranep Agar Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KSCN................................................................25.5g

Agar ..................................................................18.3g

Sodium pyruvate.................................................8.2g

Pancreatic digest of gelatin.................................6.1g

LiCl.....................................................................5.1g

Mannitol..............................................................5.1g

Beef extract.........................................................3.7g

NaN3 .................................................................0.05g

Cycloheximide................................................0.041g

Egg yolk emulsion ...................................... 100.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 100.0mL of egg yolk emulsion. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the isolation and enumeration of staphylo

cocci from foods.