培养基配方

您现在的位置：网站首页 >> 培养基配方

Kligler Iron Agar

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Kligler Iron Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..............................................................20.0g

Agar ..................................................................12.0g

Lactose..............................................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Ferric citrate........................................................0.3g

Na2S2O3 ..............................................................0.3g

Phenol Red........................................................0.05g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes. Autoclave for 15 min at 15 psi pres

sure–121°C. Pour into sterile Petri dishes or leave in

tubes.

Use: For the differentiation and identification of En

terobacteriaceae based upon sugar fermentation and

hydrogen sulfide production. Sugar fermentation is

indicated by the medium turning yellow. H2S produc

tion results in the medium turning black.