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Kleb Agar (m-Kleb Agar)

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Kleb Agar

(m-Kleb Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Proteose peptone No. 3 .....................................10.0g

NaCl....................................................................5.0g

Adonitol ..............................................................5.0g

Beef extract.........................................................1.0g

Aniline Blue........................................................0.1g

Sodium lauryl sulfate..........................................0.1g

Phenol Red......................................................0.025g

Ethanol (95% solution) ................................. 20.0mL

Carbenicillin solution.................................... 10.0mL

pH 7.4 ± 0.2 at 25°C

Carbenicillin Solution:

Composition per 10.0mL:

Carbenicillin......................................................0.05g

Preparation of Carbenicillin Solution: Add

carbenicillin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept ethanol and carbenicillin solution, to distilled/

deionized water and bring volume to 970.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 20.0mL of ethanol and

10.0mL of carbenicillin solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the enumeration of bacteria from waters.