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Iron Milk Medium

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Iron Milk Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Iron filings...........................................................1.0g

Whole milk ........................................................ 1.0L

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add iron filings, which

may be small balls of steel wool, to whole milk and

bring volume to 1.0L. Mix thoroughly. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation of lactic acid bacteria. For

the cultivation and differentiation of Clostridium

species. The medium turns black if H2S is produced.

The medium turns red if acid is produced from milk

carbohydrate fermentation. Acid and gas production

are characteristic of Clostridium perfringens and

Clostridium butyricum.