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Hugh-Leifson’s Glucose Broth (HLGB) (BAM M63)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

Hugh-Leifson’s Glucose Broth

(HLGB)

(BAM M63)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................30.0g

Glucose .............................................................10.0g

Agar ....................................................................3.0g

Peptone................................................................2.0g

Yeast extract........................................................0.5g

Bromcresol Purple ..........................................0.015g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Adjust pH to 7.4. Distibute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and differentiation of bacte

ria based on their ability to ferment glucose. Bacteria

that ferment glucose turn the medium yellow.