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HPC Agar (Heterotrophic Plate Count Agar) (m-HPC Agar)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HPC Agar

(Heterotrophic Plate Count Agar)

(m-HPC Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin...............................................................25.0g

Pancreatic digest of gelatin...............................20.0g

Agar ..................................................................15.0g

Glycerol ........................................................ 10.0mL

pH 7.1 ± 0.2 at 25°C

Source: This medium is available from BD Diag

nostic Systems.

Preparation of Medium: Add components, ex

cept glycerol, to distilled/deionized water and bring

volume to 990.0mL. Mix thoroughly. Gently heat

and bring to boiling. Add glycerol. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Pour into sterile Petri dishes.

Use: For the the cultivation and enumeration of mi

croorganisms by the membrane filter method and het

erotrophic plate count technique.