培养基配方

您现在的位置：网站首页 >> 培养基配方

HiCrome E. coli Agar B (E. coli Agar B, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-22] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome E. coli Agar B

(E. coli Agar B, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Casein enzymic hydrolysate .............................20.0g

Agar ..................................................................15.0g

Bile salts mixture ................................................1.5g

X-Glucuronide ................................................0.075g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes.

Use: For the detection and enumeration of Escheri

chia coli in foods without further confirmation on

membrane filter or by indole reagent. The chro

mogenic agent X-glucuronide used in this medium

helps to detect glucuronidase activity. E. coli cells

absorb X-glucuronide and the intracellular glucu

ronidase splits the bond between the chromophore

and the glucuronide. The released chromophore

gives coloration to the colonies. Bile salts mixture in

hibits gram-positive organisms.