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HiCrome E. coli Agar A (E. coli Agar A, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-22] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome E. coli Agar A

(E. coli Agar A, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Casein enzymic hydrolysate .............................14.0g

Agar ..................................................................12.0g

Peptone, special ..................................................5.0g

NaCl....................................................................2.4g

Bile salts mixture ................................................1.5g

Na2HPO4 .............................................................1.0g

NaH2PO4 .............................................................0.6g

X-Glucuronide ................................................0.075g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Pour into sterile Petri dishes.

Use: For the detection and enumeration of Escheri

chia coli in foods without further confirmation on

membrane filter or by indole reagent. The chromoge

nic agent X-glucuronide used in this medium helps to

detect glucuronidase activity. E. coli cells absorb X

glucuronide and the intracellular glucuronidase splits

the bond between the chromophore and the glucu

ronide. The released chromophore gives coloration

to the colonies. Bile salts mixture inhibits gram-pos

itive organisms.