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Freezing Medium (BAM M30b)

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

Freezing Medium

(BAM M30b)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 11.5mL:

Bolton broth base........................................... 9.5mL

Fetal bovine serum......................................... 1.0mL

Glycerol solution ........................................... 1.0mL

Bolton Broth Base:

Composition per liter:

Peptone ............................................................ 10.0g

Lactalbumin hydrolysate ................................... 5.0g

Yeast extract....................................................... 5.0g

NaCl................................................................... 5.0g

a-ketoglutarate ................................................... 1.0g

Na-pyruvate ....................................................... 0.5g

Na-metabisulfite ................................................ 0.5g

Na2CO3 .............................................................. 0.6g

Hemin .............................................................. 0.01g

Preparation of Bolton Broth Base: Add com

ponents to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to room temperature.

Glycerol Solution:

Composition per 100.0mL:

Glycerol ........................................................... 10.0g

Preparation of Glycerol Solution: Add glycer

ol to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to room temperature.

Preparation of Medium: Aseptically combine

9.5mL Bolton broth base, 1.0mL sterile glycerol so

lution, and 1.0mL filter sterilized fetal bovine serum.

Mix thoroughly.

Use: For the preservation of Campylobacter spp.