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培养基配方

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Fraser Secondary Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl.................................................................20.0g

Na2HPO4 ..........................................................12.0g

Beef extract........................................................5.0g

Proteose peptone................................................5.0g

Pancreatic digest of casein.................................5.0g

Yeast extract.......................................................5.0g

LiCl ....................................................................3.0g

KH2PO4............................................................1.35g

Esculin ...............................................................1.0g

Acriflavin solution ....................................... 10.0mL

Ferric ammonium citrate solution................ 10.0mL

Nalidixic acid solution................................... 1.0mL

Ferric Ammonium Citrate Solution:

Composition per 10.0mL:

Ferric ammonium citrate....................................0.5g

Preparation of Ferric Ammonium Citrate

Solution: Add ferric ammonium citrate to distilled/

deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Acriflavin Solution:

Composition per 10.0mL:

Acriflavin.......................................................0.025g

Preparation of Acriflavin Solution: Add acri

flavin to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize.

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid...................................................0.04g

NaOH (0.1N solution).................................. 10.0mL

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to 10.0mL of NaOH solution. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept acriflavin solution and ferric ammonium citrate

solution, to distilled/deionized water and bring vol

ume to 980.0mL. Mix thoroughly. Gently heat and

bring to boiling. Distribute into tubes in 10.0mL vol

umes. Autoclave for 12 min at 15 psi pressure–

121°C. Cool rapidly to 25°C. Immediately prior to

inoculation, aseptically add 0.1mL of sterile acrifla

vin solution and 0.1mL of ferric ammonium citrate

solution to each tube. Mix thoroughly.

Use: For the isolation, cultivation, and enrichment of

Listeria monocytogenes from foods based on esculin

hydrolysis. Bacteria that hydrolyze esculin appear as

black colonies.

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Fraser Secondary Enrichment Broth