培养基配方

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Fay and Barry Medium

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Amino acid....................................................... 10.0g

Peptone .............................................................. 5.0g

Yeast extract....................................................... 3.0g

Bromcresol Purple solution ........................... 5.0mL

pH 5.5 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ............................................. 0.2g

Ethanol......................................................... 50.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to 50.0mL of absolute etha

nol. Add distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

The amino acid may be L-arginine, L-ornithine, or L

lysine, depending on which amino acid decarboxy

lase activity is being measured. Mix thoroughly. Dis

tribute into tubes or flasks. Autoclave for 15 min at

15 psi pressure–121°C.

Use: For the determination of decarboxylase activi

ties of Aeromonas species.