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Ethyl Violet Azide Broth (EVA Broth)

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Ethyl Violet Azide Broth

(EVA Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................20.0g

Glucose ...............................................................5.0g

NaCl....................................................................5.0g

K2HPO4...............................................................2.7g

KH2PO4...............................................................2.7g

NaN3 ...................................................................0.3g

Ethyl Violet......................................................0.5mg

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes in 10.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Use: For the isolation, cultivation, and enumeration

of enterococci. Fecal enterococci turn the medium

turbid with a purple sediment on the bottom of the

tube.