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Enterococci Broth, Chromocult (Chromocult Enterococci Broth)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Enterococci Broth, Chromocult

(Chromocult Enterococci Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone................................................................8.6g

NaCl....................................................................6.4g

Tween 80.............................................................2.2g

NaN3 ...................................................................0.6g

5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside

(X-GLU) ......................................................0.04

pH 7.5 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix well. Distribute into tubes. Autoclave for 15 min

at 15 psi pressure–121°C. The prepared broth is clear

and yellowish.

Use: For the detection of enterococci in food and

water. The sodium-azide present in this medium

largely inhibits the growth of the accompanying, and

especially the Gram-negative microbial flora while

sparing the enterococci. The reaction occurs because

the substrate X-GLU (5-bromo-4-chloro-3-indolyl-

β-D-glucopyranoside) is cleaved, stimulated by se

lected peptones, by the enzyme β-D-glucosidase

which is characteristic for enterococci. This results in

an intensive blue-green color of the broth. Azide, at

the same time, prevents a false positive result by

most other β-D-glucosidase positive bacteria. There

fore, the color change of the broth largely confirms

the presence of enterococci and group D-streptococci.