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培养基配方
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AFPA (Aspergillus flavus/parasiticus Agar)
[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]
AFPA
(Aspergillus flavus/parasiticus Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Yeast extract......................................................20.0g
Agar ..................................................................15.0g
Peptone .............................................................10.0g
Ferric ammonium citrate.....................................0.5g
Dichloran (Botran®)........................................2.0mg
pH 6.3 ± 0.2 at 25°C
Source: This medium is available as a dehydrated
powder from Oxoid.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Add 100.0mg of chloramphenicol. Auto
clave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes.
Use: For the selective isolation and enumeration of
Aspergillus flavus and Aspergillus parasiticus. Colo
nies of these fungi appear with dark yellow-orange
color on the reverse side.