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Aeromonas Medium (Ryan’s Aeromonas Medium)

[所属分类:培养基配方] [发布时间:2021-10-28] [发布人:网站管理员2] [阅读次数:] [返回]

Aeromonas Medium

(Ryan’s Aeromonas Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.5g

Na2S2O3 ..........................................................10.67g

Proteose peptone.................................................5.0g

NaCl....................................................................5.0g

Xylose ...............................................................3.75g

L-Lysine·HCl .......................................................3.5g

Yeast extract........................................................3.0g

Sorbitol................................................................3.0g

Bile salts No. 3....................................................3.0g

Inositol ................................................................2.5g

L-Arginine·HCl....................................................2.0g

Lactose................................................................1.5g

Ferric ammonium citrate.....................................0.8g

Bromthymol Blue .............................................0.04g

Thymol Blue .....................................................0.04g

Ampicillin ........................................................5.0mg

pH 8.0 ± 0.1 at 25°C

Source: This medium is available as a dehydrated

powder from Oxoid.

Preparation of Medium: Add components, ex

cept ampicillin, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Do not autoclave. Cool to 50°C

and aseptically add 5.0mg of ampicillin. Pour into

sterile Petri dishes.

Use: For the isolation and selective differentiation of

Aeromonas hydrophila and other Aeromonas species.

Aeromonas species appear as small (0.5–1.5mm),

dark green colonies with darker centers. The addition

of ampicillin improves the recovery of Aeromonas

spp. Plesiomonas spp. as well as members of the En

terobacteriaceae also grow on this medium.