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Aeromonas hydrophila Medium

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Inositol ..............................................................10.0g

Pancreatic digest of casein................................10.0g

L-Ornithine·HCl ..................................................5.0g

Proteose peptone.................................................5.0g

Agar ....................................................................3.0g

Yeast extract........................................................3.0g

Mannitol..............................................................1.0g

Ferric ammonium citrate.....................................0.5g

Na2S2O3·5H2O ....................................................0.4g

Bromcresol Purple ............................................0.02g

pH 6.7 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat until dissolved. Adjust

pH to 6.7. Distribute into tubes in 5.0mL volumes.

Autoclave for 12 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Aeromonas

hydrophila.