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Aeromonas Differential Agar (Dextrin Fuchsin Sulfite Agar)

[所属分类:培养基配方] [发布时间:2021-10-27] [发布人:网站管理员2] [阅读次数:] [返回]

Aeromonas Differential Agar

(Dextrin Fuchsin Sulfite Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Dextrin ..............................................................15.0g

Agar ..................................................................13.0g

Pancreatic digest of casein................................10.0g

Na2HPO4...........................................................7.75g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Na2SO3................................................................1.6g

Acid Fuchsin solution................................... 50.0mL

pH 7.5 ± 0.2 at 25°C

Acid Fuchsin Solution:

Composition per 50.0mL:

Acid Fuchsin.....................................................0.25g

Preparation of Acid Fuchsin Solution: Add

Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix

well to dissolve.

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contamination of the skin.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the isolation and differentiation of Aeromo

nas species from other Gram-negative rods such as

Pseudomonas and Enterobacteriaceae. Specimens

with low numbers of Aeromonas may first be en

riched by growth in starch broth for 4–9 days. After

24 hrs of growth on this agar, colonies are sprayed

with Nadi reagent (1% solution of N,N,N′,N′-tetram

ethyl-p-phenylene-diammonium dichloride). A posi

tive Nadi reaction (dextrin degradation) is indicated

by a purple color at the periphery of the colony. Dex

trin fermentation is also indicated by red colonies.

Aeromonas species appear as large, convex, dark red

colonies with a purple periphery.