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XLD Agar (Xylose Lysine Deoxycholate Agar)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

XLD Agar

(Xylose Lysine Deoxycholate Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.5g

Lactose.......................................................................................... 7.5g

Sucrose.......................................................................................... 7.5g

Na2S2O3 ........................................................................................ 6.8g

L-Lysine......................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Xylose........................................................................................... 3.5g

Yeast extract.................................................................................. 3.0g

Sodium desoxycholate .................................................................. 2.5g

Ferric ammonium citrate............................................................... 0.8g

Phenol Red.................................................................................. 0.08g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Do not overheat. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or

leave in tubes. Plates should be poured as soon as possible to avoid pre

cipitation.

Use: For the isolation and differentiation of enteric pathogens, espe

cially Shigella and Providencia species. Nonfermenting xylose/lac

tose/sucrose bacteria appear as red colonies. Xylose-fermenting, lysine

decarboxylating bacteria appear as red colonies. Xylose-fermenting,

lysine-nondecarboxylating bacteria appear as opaque yellow colonies.

Lactose- or sucrose-fermenting bacteria appear as yellow colonies.