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XL Agar Base

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

XL Agar Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar .............................................................................................. 15 g

Lactose.......................................................................................... 7.5g

Sucrose.......................................................................................... 7.5g

L-Lysine......................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Xylose......................................................................................... 3.75g

Yeast extract.................................................................................. 3.0g

Phenol Red.................................................................................. 0.08g

Thiosulfate-citrate solution......................................................20.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Thiosulfate-Citrate Solution:

Composition per 100.0mL:

Na2S2O3 ...................................................................................... 34.0g

Ferric ammonium citrate............................................................... 4.0g

Preparation of Thiosulfate-Citrate Solution: Add components

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly.

Preparation of Medium: Add components, except thiosulfate-cit

rate solution, to distilled/deionized water and bring volume to

980.0mL. Mix thoroughly. Gently heat while stirring and bring to boil

ing. Distribute into tubes or flasks. Autoclave for 10 min at 14 psi pres

sure–118°C. Cool to 55°C. Aseptically add 20.0 mL of the sterile thio

sulfate-citrate solution. Mix thoroughly. Pour into sterile Petri dishes or

leave in tubes.

Use: For the isolation, cultivation, and differentiation of enteric patho

gens. Nonfermenting xylose/lactose/sucrose bacteria appear as red col

onies. Xylose-fermenting, lysine-decarboxylating bacteria appear as

red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria

appear as opaque yellow colonies. Lactose- or sucrose-fermenting bac

teria appear as yellow colonies.