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Wolinella succinogenes Medium

[所属分类:培养基配方] [发布时间:2021-10-14] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

K2HPO4......................................................................................... 5.0g

Fumaric acid ................................................................................. 3.0g

Sodium formate ............................................................................ 3.0g

(NH4)2SO4 .................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

MgCl2·6H2O ................................................................................. 0.2g

FeSO4·7H2O................................................................................ 0.02g

Resazurin ................................................................................... 1.0mg

Sodium thioglycolate solution .................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Sodium Thioglycolate Solution:

Composition per 10.0mL:

Sodium thioglycolate.................................................................... 0.5g

Preparation of Sodium Thioglycolate Solution: Add sodium

thioglycolate to distilled/deionized water and bring volume to 10.0mL.

Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi

pressure–121°C.

Preparation of Medium: Add components, except sodium thiogly

colate solution, to distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 10.0mL of sterile sodium thioglycolate solu

tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Wolinella succinogenes.