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Wolin-Bevis Medium

[所属分类:培养基配方] [发布时间:2021-10-14] [发布人:网站管理员2] [阅读次数:] [返回]

Wolin-Bevis Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

(NH4)2SO4 .................................................................................... 1.0g

KH2HPO4...................................................................................... 1.0g

Glucose ....................................................................................... 0.25g

L-Histidine·HCl ........................................................................... 0.25g

Tween™ 80 (polysorbate 80) ....................................................3.0mL

pH 5.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the enhanced production of chlamydospores by Candida

albicans.