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Wickerham Broth

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

Wickerham Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

KNO3 .......................................................................................... 0.78g

Yeast carbon base...................................................................100.0mL

pH 4.5 ± 0.2 at 25°C

Yeast Carbon Base:

Composition per liter:

Glucose ....................................................................................... 10.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.5g

NaCl .............................................................................................. 0.1g

CaCl2·2H2O................................................................................... 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol .......................................................................................2.0mg

H3BO3 ........................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.4mg

MnSO4·4H2O .............................................................................0.4mg

Thiamine·HCl ............................................................................0.4mg

Pyridoxine..................................................................................0.4mg

Niacin.........................................................................................0.4mg

Calcium pantothenate ................................................................0.4mg

p-Aminobenzoic acid.................................................................0.2mg

Riboflavin ..................................................................................0.2mg

FeCl3 ..........................................................................................0.2mg

Na2MoO4·4H2O .........................................................................0.2mg

KI ...............................................................................................0.1mg

CuSO4·5H2O ............................................................................0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

Preparation of Yeast Carbon Base: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: To 100.0mL of yeast carbon base, add

0.78g of KNO3 (or peptone). Mix thoroughly. Filter sterilize. Asepti

cally distribute 0.5mL into tubes containing 4.5mL of sterile distilled/

deionized water.

Use: For the cultivation and differentiation of bacteria based on nitrate

assimilation.