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Wickerham Broth

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

Wickerham Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Carbohydrate............................................................................... 10.0g

Yeast nitrogen base ................................................................100.0mL

Yeast Nitrogen Base, 10X:

Composition per liter:

Glucose ....................................................................................... 10.0g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.5g

NaCl.............................................................................................. 0.1g

CaCl2·2H2O .................................................................................. 0.1g

DL-Methionine............................................................................. 0.02g

DL-Tryptophan............................................................................. 0.02g

L-Histidine·HCl ........................................................................... 0.01g

Inositol ....................................................................................... 2.0mg

H3BO3 ........................................................................................ 0.5mg

ZnSO4·7H2O .............................................................................. 0.4mg

MnSO4·4H2O............................................................................. 0.4mg

Thiamine·HCl ............................................................................ 0.4mg

Pyridoxine.................................................................................. 0.4mg

Niacin......................................................................................... 0.4mg

Calcium pantothenate ................................................................ 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Riboflavin .................................................................................. 0.2mg

FeCl3 .......................................................................................... 0.2mg

Na2MoO4·4H2O ......................................................................... 0.2mg

KI ............................................................................................... 0.1mg

CuSO4·5H2O............................................................................ 0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

pH 4.5 ± 0.2 at 25°C

Preparation of Yeast Nitrogen Base: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: To 100.0mL of yeast nitrogen base, add

10.0g of carbohydrate. Mix thoroughly. Filter sterilize. Aseptically dis

tribute 0.5mL into tubes containing 4.5mL of sterile, distilled/deion

ized water.

Use: For the cultivation and differentiation of bacteria based on carbo

hydrate assimilation.