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Vogel S Medium for Slime-Like Neurospora

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

Vogel S Medium

for Slime-Like Neurospora

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Yeast extract................................................................................ 0.75g

Pancreatic digest of gelatin ........................................................... 0.5g

Beef extract ................................................................................... 0.3g

Vogel N 10X solution...............................................................10.0mL

Sorbitol.......................................................................................7.5mL

Vogel N 10X Solution:

Composition per 100.0mL:

Sucrose........................................................................................ 15.0g

KH2PO4 ........................................................................................ 5.0g

Trisodium phosphate..................................................................... 3.0g

MgSO4·7H2O................................................................................ 0.2g

CaCl2·H2O solution..................................................................20.0mL

Biotin solution............................................................................5.0mL

Trace elements solution .............................................................5.0mL

Preparation of Vogel N Solution: Add components, except biotin

solution and trace elements solution, to distilled/deionized water and

bring volume to 70.0mL. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Aseptically add 5.0 mL of sterile biotin solution

and 5.0mL of sterile trace elements solution. Mix thoroughly. Asepti

cally distribute into sterile tubes or flasks.

CaCl2·H2O Solution:

Composition per 20.0mL:

CaCl2·H2O .................................................................................... 0.1g

Preparation of CaCl2·H2O Solution: Add CaCl2·H2O to dis

tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.

Biotin Solution:

Composition per 100.0mL:

Biotin .........................................................................................5.0mg

Preparation of Biotin Solution: Add biotin to 50% ethanol and

bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Store at 5°C.

Trace Elements Solution:

Composition per 100.0mL:

Citric acid·H2O ............................................................................. 5.0g

ZnSO4·7H2O................................................................................. 5.0g

Fe(NH4)2(SO4)2·6H2O ................................................................. 1.0g

CuSO4·5H2O............................................................................... 0.25g

H3BO3, anhydrous...................................................................... 0.05g

MnSO4·H2O................................................................................ 0.05g

Na2MoO4·2H2O.......................................................................... 0.05g

Preparation of Trace Elements Solutions: Add components suc

cessively to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly after addition of each component. Filter sterilize. Add 2–3mL

of chloroform as a preservative. Store at 25°C.

Preparation of Medium: Add components, except Vogel N 10X

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asep

tically add 10.0mL of sterile Vogel N 10X solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Neurospora species.

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Vogel S Medium for Slime-Like Neurospora