培养基配方

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Vogel-Johnson Agar Base, HiVeg

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 16.0g

K2HPO4......................................................................................... 5.0g

Glycine........................................................................................ 10.0g

Plant hydrolysate ........................................................................ 10.0g

Mannitol...................................................................................... 10.0g

LiCl............................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

Phenol Red................................................................................ 0.025g

K2TeO3 solution.......................................................................20.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium, without tellurite, is available as a premixed

powder from HiMedia.

Caution: Lithium chloride is harmful. Avoid bodily contact and inha

lation of vapours. On contact with skin wash with plenty of water im

mediately.

K2TeO3 Solution:

Composition per 100.0mL:

K2TeO3 .......................................................................................... 1.0g

Preparation of K2TeO3 Solution: Add K2TeO3 to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components, except K2TeO3 solution,

to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile K2TeO3 solu

tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the detection of coagulase-positive Staphylococcus aureus.