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VL Agar with Blood

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

VL Agar with Blood

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Tryptone...................................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

Beef extract ................................................................................... 2.0g

Glucose ......................................................................................... 2.0g

L-Cysteine·HCl.............................................................................. 0.3g

Sheep blood or horse blood ...................................................100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Adjust pH to 7.4. Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C. Warm sheep blood to

50°C. Aseptically add 100.0mL of sterile sheep blood or 100.0mL of

sterile horse blood. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation and maintenance of Bacterionema helco

genes, Bacteroides nodosus, Bacteroides pyogenes, Bacteroides sali

vosus, Bacteroides suis, Bifidobacterium adolescentis, Bifidobacte

rium bifidum, Bifidobacterium breve, Bifidobacterium longum,

Campylobacter coli, Campylobacter concisus, Campylobacter fetus,

Campylobacter hyointestinalis, Campylobacter jejuni, Campylobacter

lari, Campylobacter mucosalis, Campylobacter species, Campy

lobacter sputorum, Capnocytophaga gingivalis, Capnocytophaga

ochracea, Capnocytophaga sputigena, Clostridium colinum, Clostrid

ium difficile, Clostridium species, Clostridium spiroforme, Falcivibrio

grandis, Falcivibrio vaginalis, Fusobacterium simiae, Gardnerella

vaginalis, Leptotrichia buccalis, Pectinatus frisingensis, Peptostrepto

coccus anaerobius, Peptostreptococcus asaccharolyticus, Peptostrep

tococcus indolicus, Peptostreptococcus magnus, Peptostreptococcus

micros, Peptostreptococcus prevotii, Peptostreptococcus tetradius,

Propionibacterium acnes, Propionibacterium avidum, Propionibacte

rium granulosum, Propionibacterium lymphophilum, and Tonsillophi

lus suis.