Anaerobic LKV Blood Agar

[所属分类:培养基配方] [发布时间:2019-10-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic LKV Blood Agar
Composition per liter:
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................13.0g
Peptic digest of animal tissue .....................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Glucose.........................................................................................1.0g
NaHSO 3 ........................................................................................0.1g
Sheep blood, laked...................................................................50.0mL
Antibiotic solution...................................................................10.0mL
Hemin solution...........................................................................1.0mL
Vitamin K 1 solution...................................................................1.0mL
pH 7.1–7.8 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Antibiotic Solution:
Composition per 10.0mL:
Kanamycin................................................................................0.075g
Vancomycin...............................................................................7.5mg
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................0.1g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Hemin Solution:
Composition per 100.0mL:
Hemin .........................................................................................0.01g
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Preparation of Medium: Add components—except sheep blood,
antibiotic solution, and vitamin K 1 solution—to distilled/deionized wa-
ter and bring volume to 939.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 50.0mL of sterile sheep blood, 10.0mL
of sterile antibiotic solution, and 1.0mL of sterile vitamin K 1 solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the isolation and cultivation of anaerobic Gram-negative
microorganisms, especially Bacteroides species.