Anaerobic HiVeg Agar without Dextrose

[所属分类:培养基配方] [发布时间:2019-10-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic HiVeg Agar without Dextrose
Composition per liter:
Plant hydrolysate.........................................................................17.5g
Agar ............................................................................................15.0g
NaCl..............................................................................................2.5g
Sodium thioglycolate....................................................................2.0g
Sodium formaldehyde sulfoxylate................................................1.0g
Methylene Blue..........................................................................2.0mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of a variety of anaerobic microorganisms.
With added blood for the detection of hemolytic activity of clostridia,
streptococci, and other anaerobic bacteria. With added carbohydrate
for fermentation studies.