Anaerobic D -Gluconate Medium

[所属分类:培养基配方] [发布时间:2019-10-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic  D -Gluconate Medium
Composition per liter:
Agar ............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
D- Gluconate...................................................................................4.0g
MgSO 4 ·7H 2 O................................................................................2.5g
(NH 4 ) 2 SO 4 .....................................................................................1.4g
L- Cysteine·HCl·H 2 O......................................................................1.0g
CaCl 2 ·2H 2 O.................................................................................0.15g
FeSO 4 ·7H 2 O................................................................................0.02g
Resazurin ...................................................................................1.0mg
NaHCO 3 solution.....................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
NaHCO 3 Solution:
Composition per 100.0mL:
NaHCO 3 ......................................................................................10.0g
Preparation of NaHCO 3 Solution: Add NaHCO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except NaHCO 3 solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Pre-
pare anaerobically under 100% N 2 . Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 10.0mL of the sterile NaHCO 3 solu-
tion. Mix thoroughly. Adjust pH to 7.1.
Use: For the cultivation and maintenance of microorganisms that can
utilize  D- gluconate as a carbon source, such as Bacteroides pectinophi-
lus.