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Toxoplasma Medium

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

Toxoplasma Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl .............................................................................................. 6.8g

NaHCO3 ........................................................................................ 2.2g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.4g

CaCl2............................................................................................. 0.2g

NaH2PO4·H2O........................................................................... 0.125g

Arginine .................................................................................... 0.105g

MgSO4 .......................................................................................... 0.1g

L-Cystine................................................................................... 0.024g

Glutamine ................................................................................. 0.292g

Histidine.................................................................................... 0.031g

Lysine........................................................................................ 0.058g

Isoleucine.................................................................................. 0.052g

Leucine ..................................................................................... 0.052g

Phenol Red................................................................................ 0.050g

Threonine.................................................................................. 0.048g

Valine ........................................................................................ 0.046g

Tyrosine .................................................................................... 0.036g

Phenylalanine............................................................................ 0.032g

Methionine................................................................................ 0.015g

Tryptophan................................................................................ 0.010g

Inositol ....................................................................................... 2.0mg

Choline....................................................................................... 1.0mg

Folic acid ................................................................................... 1.0mg

Nicotinamide.............................................................................. 1.0mg

Pantothenic acid......................................................................... 1.0mg

Pyridoxal·HCl ............................................................................ 1.0mg

Thiamine·HCl ............................................................................ 1.0mg

Riboflavin .................................................................................. 0.1mg

Fetal bovine serum, heat inactivated .....................................100.0mL

pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except fetal bovine se

rum, to distilled/deionized water and bring volume to 905.0mL. Mix

thoroughly. Adjust pH to 7.2–7.4. Autoclave for 15 min at 15 psi pres

sure–121°C. Aseptically add 100.0mL of sterile, heat-inactivated fetal

bovine serum. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks.

Use: For the cultivation of Toxoplasma gondii.