培养基配方

您现在的位置：网站首页 >> 培养基配方

Tomato Juice HiVeg Medium Base with Tomato Juice and Cycloheximide

[所属分类:培养基配方] [发布时间:2021-9-17] [发布人:网站管理员2] [阅读次数:] [返回]

Tomato Juice HiVeg Medium Base

with Tomato Juice and Cycloheximide

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Glucose ....................................................................................... 10.0g

Tomato juice solids, from 150.0mL.............................................. 7.5g

Yeast extract.................................................................................. 5.0g

Plant special peptone .................................................................... 5.0g

KH2PO4......................................................................................... 0.5g

CaCl2......................................................................................... 0.125g

MgSO4 ...................................................................................... 0.125g

KCl............................................................................................ 0.125g

NaCl .......................................................................................... 0.125g

Bromcresol Green ....................................................................... 0.03g

MnSO4 ........................................................................................ 0.03g

Tomato juice, canned .............................................................150.0mL

Cycloheximide solution ...........................................................10.0mL

pH 5.0 ± 0.2 at 25°C

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide .............................................................................. 0.1g

Preparation of Cycloheximide Solution: Add cycloheximide to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for

mation and inhalation.

Preparation of Medium: Add components, except cycloheximide

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile

cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the isolation and cultivation of lactobacilli.