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Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution (TC Minimal Medium Eagle with Earle BSS)

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tissue Culture Minimal Medium Eagle

with Earle Balanced Salts Solution

(TC Minimal Medium Eagle with Earle BSS)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1056.0mL:

NaCl.............................................................................................. 6.8g

Glucose ......................................................................................... 1.0g

KCl................................................................................................ 0.4g

CaCl2·2H2O .................................................................................. 0.2g

MgCl2·6H2O ................................................................................. 0.2g

NaH2PO4..................................................................................... 0.15g

L-Arginine ..................................................................................... 0.1g

L-Lysine....................................................................................... 0.06g

L-Isoleucine................................................................................. 0.05g

L-Leucine .................................................................................... 0.05g

L-Threonine................................................................................. 0.05g

L-Valine ....................................................................................... 0.05g

L-Tyrosine ................................................................................... 0.04g

L-Phenylalanine........................................................................... 0.03g

L-Histidine................................................................................... 0.03g

L-Cystine ..................................................................................... 0.02g

L-Methionine............................................................................... 0.02g

L-Tryptophan............................................................................... 0.01g

i-Inositol..................................................................................... 2.0mg

Calcium pantothenate ................................................................ 1.0mg

Choline chloride......................................................................... 1.0mg

Folic acid ................................................................................... 1.0mg

Nicotinamide.............................................................................. 1.0mg

Pyridoxal.................................................................................... 1.0mg

Thiamine·HCl ............................................................................ 1.0mg

Riboflavin .................................................................................. 0.1mg

Serum............................................................................50.0–100.0mL

Glutamine solution.....................................................................6.0mL

CaCl2·2H2O solution (optional).................................................2.0mL

pH 7.2–7.4 at 25°C

Glutamine Solution:

Composition per 100.0mL:

L-Glutamine................................................................................... 5.0g

NaCl (0.85% solution) ...........................................................100.0mL

Preparation of Glutamine Solution: Add the glutamine to the

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutamine and

serum, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter steril

ize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–

100.0mL of sterile serum. Human serum, bovine serum, horse serum,

or fetal calf serum may be used. Mix thoroughly. To grow cells in a

monolayer, aseptically add 2.0mL of a sterile 10% CaCl2·2H2O solu

tion. To grow cells in suspension, omit the CaCl2·2H2O solution.

Use: For preparation of Eagle’s minimal medium for the cultivation of

cells in monolayer or suspension in tissue culture.

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Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution (TC Minimal Medium Eagle with Earle BSS)