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Tissue Culture Medium RPMI No. 1640 (TC Medium RPMI #1640)

[所属分类:培养基配方] [发布时间:2021-9-16] [发布人:网站管理员2] [阅读次数:] [返回]

Tissue Culture Medium RPMI No. 1640

(TC Medium RPMI #1640)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 6.46g

Glucose ......................................................................................... 2.0g

NaHCO3........................................................................................ 2.0g

NaH2PO4................................................................................... 1.512g

KCl................................................................................................ 0.4g

L-Glutamine .................................................................................. 0.3g

L-Arginine ..................................................................................... 0.2g

Calcium nitrate.............................................................................. 0.1g

MgSO4·7H2O................................................................................ 0.1g

L-Asparagine ............................................................................... 0.05g

L-Cystine ..................................................................................... 0.05g

L-Isoleucine................................................................................. 0.05g

L-Leucine .................................................................................... 0.05g

L-Lysine·HCl ............................................................................... 0.04g

Inositol ...................................................................................... 0.035g

L-Serine ....................................................................................... 0.03g

Hydroxy-L-proline ...................................................................... 0.02g

L-Aspartic acid ............................................................................ 0.02g

L-Glutamic acid........................................................................... 0.02g

L-Proline...................................................................................... 0.02g

L-Threonine................................................................................. 0.02g

L-Tyrosine ................................................................................... 0.02g

L-Valine ....................................................................................... 0.02g

L-Histidine................................................................................. 0.015g

L-Methionine............................................................................. 0.015g

L-Phenylalanine......................................................................... 0.015g

Glycine........................................................................................ 0.01g

L-Tryptophan.............................................................................. 5.0mg

Phenol Red................................................................................ 5.0mg

Choline chloride......................................................................... 3.0mg

p-Aminobenzoic acid................................................................. 1.0mg

Folic acid ................................................................................... 1.0mg

Glutathione ................................................................................ 1.0mg

Nicotinamide.............................................................................. 1.0mg

Pyridoxine·HCl .......................................................................... 1.0mg

Thiamine·HCl ............................................................................ 1.0mg

Calcium pantothenate .............................................................. 0.25mg

Biotin ......................................................................................... 0.2mg

Riboflavin ..................................................................................0.2mg

Vitamin B12 ................................................................................5.0mg

Serum ............................................................................50.0–100.0mL

pH 7.2–7.4 at 25°C

Source: This medium is available as a premixed powder and solution

from BD Diagnostic Systems.

Preparation of Medium: Add components, except serum, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Asepti

cally add 50.0–100.0mL of sterile serum. Human serum, bovine serum,

horse serum, or fetal calf serum may be used. Mix thoroughly.

Use: For the cultivation of a wide variety of cell lines in tissue culture.

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Tissue Culture Medium RPMI No. 1640 (TC Medium RPMI #1640)