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Thiobacillus thioparus II Medium (DSMZ Medium 486)

[所属分类:培养基配方] [发布时间:2021-9-14] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus thioparus II Medium

(DSMZ Medium 486)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1003.0mL:

Solution A..............................................................................900.0mL

Solution B ..............................................................................100.0mL

Vitamin solution.........................................................................3.0mL

pH 7.4 ± 0.2 at 25°C

Solution A:

Composition per 900.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

NH4Cl ........................................................................................... 0.4g

Na2CO3 ......................................................................................... 0.4g

MgCl2·6H2O ................................................................................. 0.2g

Bromcresol Purple (saturated solution) .....................................2.0mL

Trace metals solution.................................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature.

Solution B:

Composition per 100.0mL:

KH2PO4......................................................................................... 2.0g

K2HPO4......................................................................................... 2.0g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.6.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temper

ature.

Trace Elements Solution:

Composition per liter:

Na2-EDTA................................................................................... 50.0g

NaOH.......................................................................................... 11.0g

ZnSO4·7H2O ............................................................................... 11.0g

CaCl2·2H2O ................................................................................ 7.34g

FeSO4·7H2O.................................................................................. 5.0g

MnCl2·4H2O ................................................................................. 2.5g

CoCl2·6H2O .................................................................................. 0.5g

(NH4)6Mo7O24·4H2O .................................................................... 0.5g

CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution:

Composition per liter:

Riboflavin ................................................................................20.0mg

Ca-pantothenate .......................................................................20.0mg

Nicotinic acid...........................................................................20.0mg

Pyridoxine-HCl........................................................................20.0mg

p-Aminobenzoic acid...............................................................10.0mg

Thiamine-HCl·2H2O................................................................10.0mg

Biotin .........................................................................................1.0mg

Vitamin B12 ................................................................................1.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Aseptically combine 100.0mL solution

A, 900.0mL solution B, and 3.0mL vitamin solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus thioparus.

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Thiobacillus thioparus II Medium (DSMZ Medium 486)