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Thiobacillus A2 Broth (T3 Broth)

[所属分类:培养基配方] [发布时间:2021-9-9] [发布人:网站管理员2] [阅读次数:] [返回]

Thiobacillus A2 Broth

(T3 Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Solution B .....................................................................................1.0L

Solution A ..............................................................................100.0mL

pH 8.5 ± 0.2 at 25°C

Solution A:

Composition per 100.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

Na2HPO4 ....................................................................................... 4.2g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 1.0g

Phenol Red (0.2% solution) .......................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 9.0.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution B:

Composition per liter:

MgSO4·7H2O ................................................................................ 0.1g

Trace metal solution...................................................................5.0mL

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 1.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Trace Metal Solution:

Composition per liter:

EDTA .......................................................................................... 50.0g

ZnSO4 ......................................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2.......................................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2........................................................................................... 1.61g

CuSO4 ......................................................................................... 1.57g

(NH4)2MoO4·4H2O ....................................................................... 1.1g

Preparation of Trace Metal Solution: Add components to dis

tilled/deionized water and bring volume to 1.0mL. Mix thoroughly.

Adjust pH to 6.0 with KOH.

Preparation of Medium: Aseptically add 100.0mL of sterile solution

A to 1.0L of sterile solution B. Mix thoroughly. Adjust pH to 8.5 if nec

essary. Distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus versutus and

other Thiobacillus species.