Anaerobaculum thermoterrenum Medium (DSMZ Medium 104a)

[所属分类:培养基配方] [发布时间:2019-10-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobaculum thermoterrenum Medium
(DSMZ Medium 104a)
Composition per liter:
Yeast extract................................................................................10.0g
NaCl..............................................................................................8.0g
Trypticase™ peptone....................................................................5.0g
Peptone..........................................................................................5.0g
Beef extract...................................................................................5.0g
Glucose .........................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
L -Cysteine·HCl..............................................................................0.5g
Resazurin ...................................................................................1.0mg
Na 2 S·9H 2 O solution.................................................................50.0mL
Salt solution .............................................................................40.0mL
Glucose solution ......................................................................10.0mL
pH 7.0± 0.2 at 25°C
Salt Solution:
Composition per liter:
NaHCO 3 ......................................................................................10.0g
NaCl..............................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O ................................................................................0.25g
Preparation of Salt Solution:  Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Na 2 S·9H 2 O Solution:
Composition per 100.0mL:
Na 2 S·9H 2 O....................................................................................5.0g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 100.0mL. Sparge with N 2 .
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store an-
aerobically.
Glucose Solution:
Composition per 100.0mL:
D- Glucose....................................................................................10.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with N 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Store anaerobically.
Preparation of Medium: Add components, except  L -cysteine·HCl,
glucose solution, and Na 2 S·9H 2 O solution, to distilled/deionized water
and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring
to boiling. Sparge with CO 2 . Add  L -cysteine·HCl. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL
sterile Na 2 S·9H 2 O solution and 10.0mL sterile glucose solution. Mix
thoroughly. Adjust pH to 7.0 with 8N NaOH. Distribute into sterile
tubes or flasks under anaerobic N 2 .
Use: For the cultivation and maintenance of Anaerobaculum thermoter-
renum.