AMS Agar without Methanol (Ammonium Mineral Salts Agar without Methanol)

[所属分类:培养基配方] [发布时间:2019-10-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AMS Agar without Methanol
(Ammonium Mineral Salts Agar without Methanol)

Composition per liter:
Agar ............................................................................................15.0g
MgSO 4 ·7H 2 O................................................................................1.0g
K 2 HPO 4 .........................................................................................0.7g
KH 2 PO 4 .......................................................................................0.54g
NH 4 Cl ...........................................................................................0.5g
CaCl 2 ·2H 2 O...................................................................................0.2g
FeSO 4 ·7H 2 O...............................................................................4.0mg
H 3 BO 4 ........................................................................................0.3mg
CoCl 2 ·6H 2 O...............................................................................0.2mg
ZnSO 4 ·7H 2 O..............................................................................0.1mg
Na 2 MoO 4 ·2H 2 O.......................................................................0.06mg
MnCl 2 ·4H 2 O ............................................................................0.03mg
NiCl 2 ·6H 2 O..............................................................................0.02mg
CuCl 2 ·2H 2 O.............................................................................0.01mg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Methylosinus trichospo-
rium and other methane-oxidizing bacteria. Cultures are grown under
an atmosphere of 50% methane.